The head of the group is Galina A. Klyasova (pictured).
The department was established in 2000. The main purpose of the laboratory is the diagnosis of bacterial and fungal infections.
Scientific and clinical work in the laboratory is carried out in several areas: 1) diagnosis, treatment and prevention of infections in patients with hemoblastosis, 2) organization of multicenter studies in Russia to study the etiology of sepsis and the sensitivity of isolated microorganisms to antimicrobial drugs in oncohematology, 3) development of protocols aimed at introducing new methods for the diagnosis and treatment of infectious complications in patients with tumors of the blood system, 4) conducting molecular genetic studies of hospital microorganisms in order to determine the pathways of infection within the hospital. The result of ongoing scientific research was a decrease in mortality from severe infectious complications such as sepsis and invasive aspergillosis in patients with hematological malignancies.
Scientific work is routinely paired with medical, advisory and pedagogical practice - these are training sessions on infectious complications in hematology, training of clinical residents and graduate students, lecturing students on the diagnosis and treatment of infections in oncohematology, and training doctors from other laboratories on laboratory diagnostics.
The department conducts a full range of studies on the diagnosis of infections caused by bacteria and fungi, including yeasts and molds. In order to detect fungi, fluorescent microscopy, culture studies (candida, cryptococcus, aspergillus, mucormycetes, pneumocystis, and others), serological studies (determination of antigen and Candida antibodies, determination of cryptococcus antigen, determination of Aspergillus antigen) are carried out.
This year, studies have begun on the isolation of legionella culture from patient samples.
The department has a powerful material and technical base to perform all the necessary bacteriological and mycological studies for patients. It is equipped with the necessary modern automatic analyzers, which make it possible to receive results in the shortest possible amount of time.
Department staff. First row (left to right): senior researcher, A.V. Mironova; laboratory assistant N.N. Aleshkina. Second row (from left to right): laboratory assistant, A.I. Kretsul; doctor, L.N. Shlykova; researcher, A.N. Gracheva; head of the laboratory, G.A. Klyasova; laboratory assistant, E.A. Egorova; doctor, A.O. Bogdanova; laboratory assistant, T.D. Goncharova; laboratory assistant, Z.M. Zakharova.Third row (from left to right): laboratory assistant, E.N. Golubeva; Cand. Sci. (Med.), A.G. Korobova; Cand. Sci. (Med.), V.A. Okhmat
The department conducts research on the diagnosis of bacterial and fungal infections, microbiological research.
Key department staff
Anastasia V. Mironova — senior researcher. Conducts a complex of studies related to the resistance of bacteria to antimicrobial drugs. For the first time in Russia, Mironova isolated enterococcus resistant to vancomycin, and together with graduate student A.N. Brilliantova, sequenced the isolated strains, which confirmed their belonging to an epidemic complex capable of causing outbreaks of infections in a hospital.
Alexandra N. Gracheva is one of the leading specialists in Russia in the diagnosis of infections caused by fungi, both yeast and mold. She supervises multicenter studies on the etiological structure of invasive mycoses in Russia.
Irina N. Frolova - conducts serological tests for the diagnosis of fungal infections.
Department equipment
Desktop MALDI-TOF Microflex mass spectrometer (Bruker Daltonics, USA). Allows fast and accurate verification of bacteria and fungi. Identification of microorganisms takes only a few minutes.
The latest generation automatic system for cultivation of microorganisms from blood BACTEC FX (BECTON DICKINSON, USA). Designed for the rapid cultivation of bacteria and fungi in blood samples.
Automatic system for determining the sensitivity of bacteria and yeast to antimicrobials VITEC 2 Compact (BIOMERIEUX, France). Sensitivity to antimicrobial drugs is determined with the indication of the minimum inhibitory concentration (MIC), the identification of resistance mechanisms.
Equipment for ELISA
Reader for express diagnostics Quantum Blue (Bühlmann, Switzerland). Designed for quantitative express determination of calprotectin in stool samples by lateral immunochromatography.
Washing device PW 40 (Bio-Rad, USA).
